Custom Peptide Services

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We manufacture high quality custom peptides tailored for your particular needs!

With strong roots in the scientific area and longtime experience in peptide synthesis, Intavis supports you in areas like signal transduction, proteomics, epigenetics, immunology (e.g. epitope mapping) and many more.

We cover a wide range of applications: From the use of purified peptides for quantitative studies, fluorescently labeled peptides for localization assays, peptide libraries for economic screening purposes and CelluSpots™ peptide arrays that are based on SPOT-synthesis, Intavis peptide services offer TCP-resins for solid phase peptide synthesis.

Custom peptide synthesis, typically provided for peptides with a length between 5 and 60 residues, is carried out on state-of-the-art fully automated instruments on solid phase. All peptides are HPLC purified, analysed by MALDI-TOF mass spectrometry and shipped with detailed quality control documentation including HPLC and MS spectra. Quantities usually range from 1mg to gram scale at a purity of up to 98% (HPLC). Peptides are usually synthesized with free N-terminal amino and C-terminal carboxy groups, but modifications such as acetylation, biotinylation or amidation are available as well as synthesis of isotopic labeled peptides for quantitative proteomic studies. Cyclisation via disulfide bond formation, modifications or special amino acids (i.e. phosphorylated, myristoylated, D-amino acids, fluorescent labels etc.) are routinely available upon request to suit your requirements.

Peptide Libraries

Peptide libraries are efficient tools for screening of epitopes, proteases, kinases, antibodies, pharmacologically active peptides and to address many other biochemical or immunological questions. In many cases, the use of peptide libraries is an economic alternative to recombinant protein approaches. Our peptide libraries consist of unpurified peptides with different levels of quality control, delivered in a 96-tube rack format. Purified peptide libraries are available on request.

Custom Peptide Sets for T-Cell Stimulation/Epitope Mapping

Overlapping peptides have been widely used to map epitopes that simulate T-lymphocytes in vitro. Synthetic peptides bind to a groove of extracellular MHC molecules, presented on the surface of cells. CD4 T-cell receptors interact with MHC class II complexes and CD8 T-cells receptors with MHC class I complexes. The binding groove MHC class II molecules has open ends, making it accessible for peptides with a length of >10 amino acid residues, whereas the binding pocket MHC class I molecules has closed ends that only allow 8-10 amino acid long peptides to enter. However, it was shown that 15 amino acid long peptides can efficiently stimulate both CD4 and CD8 T-cells.

Spot-membrane Peptide Array

SPOT synthesis technique enables low-cost, fast and accurate synthesis of customized peptide sequences or other compounds arrayed on cellulose membrane. INTAVIS offers SPOT-membrane Peptide Arrays ready to use in your screening or binding experiments. Peptides are synthesized on a modified cellulose support with flexible PEG spacers between the membrane and the covalently linked peptides. The cellulose support is perfectly suited for Western BLOT-like incubation and detection experiments. Unusual and modified amino acids as well as standard proteinogenic amino acids may be used in the custom peptide array. Several applications of the SPOT-synthesis method have been described in a large number of scientific publications.

CelluSpots™ – Custom Peptide Arrays

CelluSpots™ are arrays of peptide-cellulose conjugates spotted on planar surfaces such as glass slides. Peptides are synthesized on a modified cellulose support which can be dissolved after synthesis. The solutions of individual peptides covalently linked to macro molecular cellulose are then spotted onto a surface of choice. After evaporation of the solvent a three-dimensional layer is formed which is not dissolved in aqueous reagents used for standard assays. The three-dimensional structure holds up to 1000 times more peptides per area as compared to conventional monolayer deposition.

CelluSpots™ – Kinase Substrate Arrays

Ready to screen kinase arrays with tyrosine- and serine/threonine-kinase substrates from annotated phosphorylation sites. Three different arrays, each with 384 spots in duplicate surrounded by red colour marks, are available. These arrays are perfect tools to characterize substrate specificities of kinases, to compare kinases, to identify autophosphorylation sites or to analyze kinase inhibitors. Beside annotated sequences the arrays contain peptide mixtures with defined fixed positions represented by one or a few amino acids only. These mixtures can help to profile substrate specificities of unknown or new kinases.

Mass Spectrometry Standards

Mass Spectrometry Standards are used to calibrate Mass Spectrometers on a regular basis.
While originally provided by Mass Spectrometer Manufacturers, supply eventually came to a stop.
The classical standards were also comprised of randomly chosen peptides that proved less than ideal.

Intavis offers two Mass Spectrometry Standards.

Synthesis Resin

We offer preloaded TCP resin as well as Rink-Amide-resin for the routine production of peptide acids and amides. We use these high quality resins for our own production for most of our custom peptide synthesis. Both type of resins are ideally suited for solid phase peptide synthesis following the Fmoc strategy.