DigestPro MSi

Automated protein digestion and sample preparation

The DigestPro MSi automates all of the tedious manual steps of in-gel or in-solution digestion protocols:

• • reduction of disulfide bonds at 60°C
  • alkylation of cysteine residues
  • enzymatic digestion at 37°C
  • washing of gel slices and elution of the peptides from the gel matrix

In addition, various procedures for subsequent sample preparation can be automated:

• • desalting and concentration with Millipore® ZipTips®
  • handling of other micro-extraction tips for desalting, enrichment and affinity purification
  • use of StageTips
  • MALDI target spotting on targets of all manufacturers
  • preparation of sandwich spots
  • loading of samples on Bruker Anchor plates

The DigestPro MSi offers the following features

• • modular work area setup:
  • freely adjust the instrument according to your individual needs
  • automation of different applications on one platform
  • freely selectable temperature for each step
  • new cooling option for eluted peptides or in solution digestion
  • user friendly software for free adaptation of individual protocols
  • use of standard lab buffer (low running costs)
  • compact size