Synthetic peptides have become important tools in many areas of molecular biology. Even long sequences can be synthesized quickly and with high quality using the ResPep CF. The real-time UV detection system monitors the Fmoc-cleavage reactions and automatically extends the deprotection and coupling times in difficult regions. An elongated or double coupling step can follow multiple deprotections before the system proceeds with the next cycle. The user can program the synthesizer to perform more complex operations based on the monitoring data. Other choices could be multiple couplings, changing coupling conditions or reducing the number of washing steps needed. This leads to a greater coupling yield and superior peptides and also saves time and reduces reagent waste.