Several applications of the SPOT synthesis method have been described in a large number of scientific papers. The applications can be grouped in categories such as:
- Epitope mapping and characterization
- Analysis of kinase substrates
- Metal binding analysis
- Receptor binding studies
- Peptide Nucleic Acid arrays (PNA)
More than 400 scientific articles document the power of the SPOT method for analysis of protein-protein interaction. Handling of the covalently bound peptide arrays is easy and compatible with techniques available in most molecular biology laboratories.