Plate and Mini-Column Module
In the ResPep SL base configuration up to 96 peptides are made in a 96 well filter plate filled with synthesis support. Alternatively the microcolumn module can be used to synthesize up to 24 peptides or PNAs at a slightly larger scale. Reagents are held in the reaction wells and are extracted by vacuum applied to the bottom of the reaction module. An 4-channel manifold connected to a solvent pump is used for rapid washing. The chemistry is optimized for Fmoc-based peptide synthesis with activation by HCTU/NMM, HBTU/DIPEA or a similar protocol. If larger amounts of peptides are needed, an optional Column Module can be used to make up to 3 peptides at a scale ranging from 25-100 µmol.