Links and References

InsituPro VSi- Next generation in situ hybridization and immunohistochemistry

Selected reference articles and useful resources for the InsituPro product line
Please contact us for additional publications.

An optimised whole mount in situ hybridisation protocol for the mollusc Lymnaea stagnalis
Hohagen, J. et al., BMC Developmental Biology, 2015, 15, 19

ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled.
Miyatake, K. et al., Nat Commun, 2015, 6, 6666

mab21-l3 regulates cell fate specification of multiciliate cells and ionocytes Takahashi, C.; et al., Nat Commun, 2015, 6, 6017

SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice.
Wu, Q. et al., Development, 2015, 142, 575-586

Β-catenin-dependent control of positional information along the AP body axis in planarians involves a teashirt family member.
Reuter, H. et al. Cell Rep. 2015, 10(2): 253-65.

D6 PROTEIN KINASE Activates Auxin Transport-Dependent Growth and PIN-FORMED Phosphorylation at the Plasma Membrane.
Barbosa, I. C. R. et al., Dev Cell, 2014, 29, 674-685

A mutation in the enzyme monoamine oxidase explains part of the Astyanax cavefish behavioural syndrome.
Elipot, Y. et al., Nat Commun, 2014, 5, 3647

The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants.
Gadeyne, A. et al., Cell, 2014, 156, 691-704

Simplet/Fam53b is required for Wnt signal transduction by regulating $-catenin nuclear localization.
Kizil, C. et al., Development, 2014, 141, 3529-3539

In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration.
Choi, W.-Y. et al., Development, 2013, 140, 660-666

A small molecule screen identifies a novel compound that induces a homeotic transformation in Hydra.
Glauber, K. M.  et al, Development, 2013, 140, 4788-4796

Sox2 marks epithelial competence to generate teeth in mammals and reptiles.
Juuri, E. et al., Development, 2013, 140, 1424-1432

Circular RNAs are a large class of animal RNAs with regulatory potency.
Memczak, S. et al., Nature, 2013, 495, 333-338

Follistatin antagonizes activin signaling and acts with notum to direct planarian head regeneration.
Roberts-Galbraith, R. H. & Newmark, P. A., PNAS, 2013, 110, 1363-1368

Two direct targets of cytokinin signaling regulate symbiotic nodulation in Medicago truncatula.
Ariel, F. et al., Plant Cell, 2012, 24, 3838-3852

A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants.
Barbez, E.; et al., Nature, 2012, 485, 119-122

Regenerative neurogenesis from neural progenitor cells requires injury-induced expression of Gata3.
Kizil, C. et al., Dev Cell, 2012, 23, 1230-1237

miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis.
Rebustini, I. T. et al., Development, 2012, 139, 191-202

Polarized cell growth in Arabidopsis requires endosomal recycling mediated by GBF1-related ARF exchange factors.
Richter, S. et al., Nat Cell Biol, 2012, 14, 80-86

Jagged 1 regulates the restriction of Sox2 expression in the developing chicken inner ear: a mechanism for sensory organ specification.
Neves, J. et al., Development, 2011, 138, 735-744

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos.
Geetha-Loganathan, P. et al., Dev Dyn, 2011, 240, 2108-2119

Rhou maintains the epithelial architecture and facilitates differentiation of the foregut endoderm.
Loebel, D. A. F. et al., Development, 2011, 138, 4511-4522

Msx genes define a population of mural cell precursors required for head blood vessel maturation.
Lopes, M. et al., Development, 2011, 138, 3055-3066

Noggin and noggin-like genes control dorsoventral axis regeneration in planarians.
Molina, M. D. et al., Curr Biol, 2011, 21, 300-305

Id gene regulation and function in the prosensory domains of the chicken inner ear: a link between Bmp signaling and Atoh1.
Kamaid, A. et al., J Neurosci, 2010, 30, 11426-11434

Arabidopsis Tyrosylprotein sulfotransferase acts in the auxin/PLETHORA pathway in regulating postembryonic maintenance of the root stem cell niche.
Zhou, W. et al., Plant Cell, 2010, 22, 3692-3709

The RNA binding protein Tudor-SN is essential for stress tolerance and stabilizes levels of stress-responsive mRNAs encoding secreted proteins in Arabidopsis.
Frei dit Frey, N. et al, Plant Cell, 2010, 22, 1575-1591

Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation.
Sun, J. et al., Plant Cell, 2009, 21, 1495-1511

ENDOSPERM DEFECTIVE1 Is a Novel Microtubule-Associated Protein Essential for Seed Development in Arabidopsis.
Pignocchi, C. et al., Plant Cell, 2009, 21, 90-105

Similar regulatory logic in Ciona intestinalis for two Wnt pathway modulators, ROR and SFRP-1/5.
Auger, Hé. et al., Dev Biol, 2009, 329, 364-373

Rainbow Trout Gonadal Masculinization Induced by Inhibition of Estrogen Synthesis Is More Physiological Than Masculinization Induced by Androgen Supplementation
Vizziano et al., Biology of reproduction 2008, in press

Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants
Huang et al., BMC Developmental Biology 2008, 8:31

Edar and Troy signalling pathways act redundantly to regulate initiation of hair follicle development
Pispa et al., Human Molecular Genetics 2008, 17(21):3380-3391

Functional diversification of closely related ARF-GEFs in protein secretion and recycling
Richter et al., Nature 2007 448, 488-492

Wurst is essential for airway clearance and respiratory-tube size control
Behr et al., Nature Cell Biology 2007, 9(7):847-53.

BMP signaling regulates the dorsal planarian midline and is needed for asymmetric regeneration
Reddien et al., Development 2007, 134, 4043-4051

Wnt signals provide a timing mechanism for the FGF-retinoid differentiation switch during vertebrate body axis extension
Olivera-Martinez and Storey, Development 2007, 134, 2125-2135

Gene Duplication of the Zebrafish kit ligand and Partitioning of Melanocyte Development Functions
Hultman et al., PLoS Genet 2007, 3(1): e17

Arabidopsis JAGGED LATERAL ORGANS Is Expressed in Boundaries and Coordinates KNOX and PIN Activity
Borghi et al., The Plant Cell 2007, 19:1795-1808

Expanded expression of Sonic Hedgehog in Astyanax cavefish: multiple consequences on forebrain development and evolution
Menuet et al., Development 2007, 134, 845-855

Regeneration and maintenance of the planarian midline is regulated by a slit orthologue
Cebria et al., Dev Biol 2007, 307 (2), 394-406

BMP signaling regulates the dorsal planarian midline and is needed for asymmetric regeneration
Reddien et al., Development 2007, 134, 4043-4051

In situ hybridisation techniques for mRNA detection in whole mount Arabidopsis samples
Hejatko et al., Nat. Protoc. 2006; 1(4): 1939-1946
“Our protocol was adopted for automation using an in situ dedicated robot “InsituPro” from INTAVIS. To create a program to perform the procedure according to our protocol is rather simple as the manufacturer provides instructions for very intuitive and user friendly programming of the automate. Using this robot […] tissue permeabilisation, hybridisation and washing steps until detection […] can be performed.”

Dynamic and Compensatory Responses of Arabidopsis Shoot and Floral Meristems to CLV3 Signaling
Müller et al., The Plant Cell 2006, 18, 1188-1198

The molecular setup of the avian head mesoderm and its implication for craniofacial myogenesis
Bothe and Dietrich, Dev. Dynamics 2006; 235, 2845-2860

Retinaldehyde dehydrogenase 2 (RALDH2)-mediated retinoic acid synthesis regulates early mouse embryonic forebrain development by controlling FGF and sonic hedgehog signaling
Ribes et al., Development 2006, 133, 351-361

her1 and her13.2 are jointly required for somitic border specification along the entire axis of the fish embryo
Sieger et al., 2006 Dev Biol 293 (1), 242-251

Comparative analysis of her genes during fish somitogenesis suggests a mouse/chick-like mode of oscillation in medaka
Gajewski et al., Dev Genes Evo 2006, 216 (6) 315-332

The orphan COUP-TF nuclear receptors are markers for neurogenesis from cnidarians to vertebrates.
Gauchat, D. et al., Dev Biol, 2004, 275, 104-123

Large-scale expression screening by automated whole-mount in situ hybridization
Quiring et al., Mechanisms of Development 2004; 121(7-8), 971-976

Nonindependence of mammalian dental characters
Kangas et al., Nature 2004, 432, 211-214

The Claudin-like Megatrachea Is Essential in Septate Junctions for the Epithelial Barrier Function in Drosophila
Behr et al., Developmental Cell, 2003; 5, 611–620

Automated whole mount localisation techniques for plant seedlings
Friml et al., 2003, Plant Journal Volume 34 Issue 1, Pages 115 – 124

Automated in situ detection (AISD) of biomolecules
Plickert et al., Dev. Genes Evol 1997; 207, 362-367


Useful resources:


emap – The Edinburgh Mouse Atlas Project
A digital atlas of mouse development and database to be a resource for spatially mapped data such as in situ gene expression and cell lineage.

GEISHA – The Gallus gallus (chicken) EST and In Situ Hybridization Analysis database
A developing data base for chick ESTs and corresponding in situ images.

ZFIN – The zebrafish model organism database
A database containing everything starting from genes, ESTs, expression data up to the request for fish lines.

MEPD – Medaka Expression Patern Data Base
A database on in situ expression pattern of ESTs in the ricefish Medaka.

CK EST expression database of Flybase
The EST in situ expression database of flybase, a resource for Drosophila.

The gene expression database of TAIR
The gene expression resource of the TAIR Arabidopsis database. Please read their guidelines before linking or citing.

Xenopus express
Supplier of Pipid Frogs for laboratory research