Does the InsituStain have to be operated in a fume hood?
No, all toxic substances are in a closed system. Nevertheless it is small enough to be easily placed in a fume hood.

What is the mesh size of the net at the bottom of the baskets?
The standard mesh size is 100 µm, baskets with 55 and 35 µm mesh sizes are available for smaller specimens.

What is the temperature range for incubations?
The thermoplate can be cooled to 4°C and heated to 80°C.

How many different buffers can be used?
You can use up to 12 different buffers in one run. However, buffer bottles can be easily exchanged during a run, extending the capacity.

Can precious solutions be cooled or preheated?
Yes, there are two positions for 50 ml Falcon tubes cooled down to 8°C permanently. In addition, there are two positions for 250 ml bottles permanently heated to 50°C.

What are the buffer volumes delivered?
Depending on the work area, the delivered buffer volumes can be 25 ml to 250 ml.

Is there any agitation during the incubation steps?
The incubation tub of the InsituStain is mounted on a shaking platform. There are three different modes possible: no shaking, interval shaking or continuous shaking.

What is the run time of a typical protocol?
The duration is comparable to a manual protocol but as the InsituStain can work during the night, it may be a little shorter.

Can the specimens be treated with individual or identical probes?
Both is possible. If treated with individual probes, the hybridization has to be performed manually, outside of the machine.