CelluSpots™ peptide arrays are a convenient tool for the detection of interaction partners with defined amino acid sequences that are presented on the chip surface:
- protein-protein interactions
- receptor-ligand interactions
- nucleic acid binding
- enzyme activities (e.g. kinases)
- epitope mapping
- metal binding studies
Pre-made Kinase Substrate Arrays
Ready to screen kinase arrays with tyrosine- and serine/threonine-kinase substrates from annotated phosphorylation sites are available now. We offer three different arrays, each with 384 spots in duplicate surrounded by red color marks. These arrays are perfect tools to characterize substrate specificities of kinases, to compare kinases, to identify autophosphorylation sites or to screen for kinase inhibitors.
Beside annotated sequences the arrays contain peptide mixtures with defined fixed positions represented by one or a few amino acids only. These mixtures can help to profile substrate specificities of unknown or new kinases.
CelluSpots™ peptide arrays provide a versatile tool that can be adapted to your research needs. Combining the well known SPOT technology with the advantages of mini- and micro-arrays, the new format is a more powerful tool than peptide libraries on cellulose membranes.
The smaller format decreases the sample volume required. Now time course studies of the immune response of a mouse become feasible.
Another advantage is the high number of identical copies of the array that can be produced. Now large series of sera can be compared in a parallel experiment, and copies of the array can be saved for confirmation experiments to be carried out years later.
Examples for two detection methods:
Fig 1: Peptide library, phospho-specific antibody incubation, detection: chemiluminescence (ECL kit).
Fig 2: Various borrelia antigens, serum incubation, detection: color development (DAB).
