Does the MultiPep have to be operated in a fume hood? No, the instrument comes with a self-contained cabinet. An exhaust tube leading to a ventilation system must be connected to an outlet provided.
How easy is a change of synthesis scale on the MultiPep? To select a different scale you have to load the correct synthesis plate and select a protocol corresponding to this scale. We supply optimized routines for several synthesis scales.
Is there any synthesis monitoring in the MultiPep? No, the MultiPep follows an optimized coupling protocol. Sequence-related problems must be deducted from the mass spectrum of the final peptide product.
Can I use bulk reagents? Yes, the MultiPep was designed for economical operation. Solvents and activator are bought from standard, recommended suppliers. Only amounts needed for a particular synthesis are weighed, dissolved and used.
Which reaction plates are delivered with the MultiPep? Our default scale is 5 µmol and we will deliver a basic set of 96 well reaction plates and amino acid vials for this scale. The plates are also usable at a scale of 1 µmol, and we provide a different synthesis plate to be used for a scale of 5-10 µmoles.
How do we fill empty synthesis plates? To fill the individual cavities of the reaction plate you make a slurry of resin and then pipet it into the wells.