Several applications of the SPOT synthesis method have been described in a large number of scientific papers. The applications can be grouped in categories such as:

• Epitope mapping and characterization
• Analysis of kinase substrates
• Metal binding analysis
• Receptor binding studies
• Peptide Nucleic Acid arrays (PNA)

More than 300 scientific articles document the power of the SPOT method for analysis of protein-protein interaction. Handling of the covalently bound peptide arrays is easy and compatible with techniques available in most molecular biology laboratories.